Part:BBa_K1966004:Experience

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Applications of BBa_K1966004

UTK's 2016 iGEM team was unable to demonstrate that the Pu promoter could be activated by the xylR-substrate complex in the presence of the substrates toluene and a xylene isomer mixture. This promoter has been shown to function in E. coli in previous literature(1), but the mechanism of activity is relatively complex and therefore the team does not know the reason it was unable to function. The figure below shows lack of expression of RFP in this part when E. coli BL21 DE3 containing BBa_K1966004 was grown in the presence of each substrate investigated. The plasmid "pSynth6" was a positive control for RFP expression containing a constituitively RFP (RFP from part BBa_J04450).

A brief schematic is shown here outlining the mechanism of activity of the Pu promoter and its activation via xylR (xylR is 
contained in this part under the control of registry's basic lac promoter).

Reference: (1) Kim, Mi Na et al. Construction and comparison of E. coli whole-cell biosensors capable of detecting aromatic
compounds. 2005. Journal of Microbiological Methods, Vol 60.

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